作为PDZ结构域配体的双价肽
Bivalent peptides as PDZ domain ligands.
作者信息
Klosi Edvin, Saro Dorina, Spaller Mark R
机构信息
Department of Chemistry, Wayne State University, Detroit, MI 48202, USA.
出版信息
Bioorg Med Chem Lett. 2007 Nov 15;17(22):6147-50. doi: 10.1016/j.bmcl.2007.09.035. Epub 2007 Sep 12.
Abstract
A series of multivalent peptides, with the ability to simultaneously bind two separate PDZ domain proteins, has been designed, synthesized, and tested by isothermal titration calorimetry (ITC). The monomer sequences, linked with succinate, varied in length from five to nine residues. The thermodynamic binding parameters, in conjunction with results from mass spectrometry, indicate that a ternary complex is formed in which each peptide arm binds two equivalents of the third PDZ domain (PDZ3) of the neuronal protein PSD-95.
摘要
已经设计、合成了一系列具有同时结合两种不同PDZ结构域蛋白能力的多价肽，并通过等温滴定量热法（ITC）进行了测试。与琥珀酸连接的单体序列长度从五个到九个残基不等。热力学结合参数与质谱结果表明形成了一种三元复合物，其中每个肽臂结合两个当量的神经元蛋白PSD-95的第三个PDZ结构域（PDZ3）。
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