利用Telospot对端粒酶调节剂进行低通量至高通量分析。

Low- to high-throughput analysis of telomerase modulators with Telospot.

作者信息

Cristofari Gaël, Reichenbach Patrick, Regamey Pierre-Olivier, Banfi Damiano, Chambon Marc, Turcatti Gerardo, Lingner Joachim

机构信息

Swiss Institute for Experimental Cancer Research, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland.

出版信息

Nat Methods. 2007 Oct;4(10):851-3. doi: 10.1038/nmeth1099.

DOI:10.1038/nmeth1099

PMID:17893679

Abstract

We designed a method termed Telospot to discover and characterize telomerase modulators as anticancer drugs or chemical biology tools. Telospot is based on a highly efficient human telomerase expression system and the detection of telomerase DNA reaction products in macroarray format. Telospot offers a highly scalable, cost- and time-effective alternative to presently available telomerase assays, which are limited by the requirement for PCR, telomerase purification or technologies not amenable to high throughput.

摘要

我们设计了一种名为端粒斑点法（Telospot）的方法，用于发现和表征作为抗癌药物或化学生物学工具的端粒酶调节剂。端粒斑点法基于一种高效的人类端粒酶表达系统，并以宏阵列形式检测端粒酶DNA反应产物。与目前可用的端粒酶检测方法相比，端粒斑点法具有高度可扩展性、成本效益高和省时的特点，目前的端粒酶检测方法受到PCR要求、端粒酶纯化或不适用于高通量技术的限制。