Andrianasolo Eric H, Haramaty Liti, Degenhardt Kurt, Mathew Robin, White Eileen, Lutz Richard, Falkowski Paul
Center for Marine Biotechnology, Institute of Marine and Coastal Sciences, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08901-8521, USA.
J Nat Prod. 2007 Oct;70(10):1551-7. doi: 10.1021/np070088v. Epub 2007 Sep 28.
Four new diterpenes ( 1- 4) were isolated from the soft coral Xenia elongata using a novel cell-based screen for apoptosis-inducing, potential anticancer compounds. The molecular structures of the diterpenes were determined using a combination of NMR and mass spectrometry. The bioactivities were confirmed using a specific apoptosis induction assay based on genetically engineered mammalian lines with differential, defined capacities for apoptosis. The diterpenes induce apoptosis in micromolar concentrations. This is the first report of apoptosis induction by marine diterpenes in xenicane skeletons.
利用一种新型的基于细胞的筛选方法，从软珊瑚长指异柳珊瑚中分离出四种新的二萜类化合物（1-4），用于筛选具有诱导凋亡潜力的抗癌化合物。通过核磁共振（NMR）和质谱联用确定了这些二萜类化合物的分子结构。使用基于具有不同、明确凋亡能力的基因工程哺乳动物细胞系的特异性凋亡诱导试验，证实了其生物活性。这些二萜类化合物在微摩尔浓度下即可诱导凋亡。这是关于具有异柳烷骨架的海洋二萜类化合物诱导凋亡的首次报道。
