CD107动员检测的应用表明，在单核细胞增生李斯特菌感染期间，具有新型MHC-Ib限制性的细胞毒性T淋巴细胞被激活。
Use of the CD107 mobilization assay reveals that cytotoxic T lymphocytes with novel MHC-Ib restriction are activated during Listeria monocytogenes infection.
作者信息
McElroy Denise S, Badstibner Adina M, D'Orazio Sarah E F
机构信息
Department of Microbiology, Immunology & Molecular Genetics, University of Kentucky College of Medicine, Lexington, KY 40536, USA.
出版信息
J Immunol Methods. 2007 Dec 1;328(1-2):45-52. doi: 10.1016/j.jim.2007.08.005. Epub 2007 Aug 30.
Abstract
Detection of cytotoxic activity by pathogen-specific T cells of unknown antigenic specificity is difficult due to the limitations of using infected cells, instead of peptide-pulsed cells, as targets. We report here that the recently described CD107 mobilization assay readily allowed for the ex vivo detection of cytotoxic T lymphocytes (CTL) with a novel MHC-Ib restriction that specifically recognized Listeria monocytogenes-infected macrophages. The CD107 mobilization assay is likely to be a useful tool for detection of CD8(+) T cells that recognize a wide variety of intracellular pathogens.
摘要
由于使用感染细胞而非肽脉冲细胞作为靶标存在局限性，因此难以检测抗原特异性未知的病原体特异性T细胞的细胞毒性活性。我们在此报告，最近描述的CD107动员试验能够轻松地在体外检测具有新型MHC-Ib限制性的细胞毒性T淋巴细胞（CTL），该CTL可特异性识别单核细胞增生李斯特菌感染的巨噬细胞。CD107动员试验可能是检测识别多种细胞内病原体的CD8(+) T细胞的有用工具。
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