一种鼠疫耶尔森菌lpxM突变体活疫苗可增强小鼠和豚鼠对腺鼠疫的免疫力。
A Yersinia pestis lpxM-mutant live vaccine induces enhanced immunity against bubonic plague in mice and guinea pigs.
作者信息
Feodorova V A, Pan'kina L N, Savostina E P, Sayapina L V, Motin V L, Dentovskaya S V, Shaikhutdinova R Z, Ivanov S A, Lindner B, Kondakova A N, Bystrova O V, Kocharova N A, Senchenkova S N, Holst O, Pier G B, Knirel Y A, Anisimov A P
机构信息
Russian State Anti-Plague Research Institute Microbe, 46 Universitetskaya Street, Saratov 410005, Russia.
出版信息
Vaccine. 2007 Nov 1;25(44):7620-8. doi: 10.1016/j.vaccine.2007.08.055. Epub 2007 Sep 14.
The lpxM mutant of the live vaccine Yersinia pestis EV NIIEG strain synthesising a less toxic penta-acylated lipopolysaccharide was found to be avirulent in mice and guinea pigs, notably showing no measurable virulence in Balb/c mice which do retain some susceptibility to the parental strain itself. Twenty-one days after a single injection of the lpxM-mutant, 85-100% protection was achieved in outbred mice and guinea pigs, whereas a 43% protection rate was achieved in Balb/c mice given single low doses (10(3) to 2.5 x 10(4) CFU) of this vaccine. A subcutaneous challenge with 2000 median lethal doses (equal to 20,000 CFU) of fully virulent Y. pestis 231 strain, is a 6-10-fold higher dose than that which the EV NIIEG itself can protect against.
研究发现，活疫苗鼠疫耶尔森菌EV NIIEG菌株的lpxM突变体合成的脂多糖毒性较低，在小鼠和豚鼠中无致病性，尤其是在对亲本菌株仍有一定易感性的Balb/c小鼠中未表现出可测量的毒力。单次注射lpxM突变体21天后，远交系小鼠和豚鼠的保护率达到85%-100%，而给予该疫苗低剂量单次注射（10³至2.5×10⁴CFU）的Balb/c小鼠的保护率为43%。用2000个半数致死剂量（相当于20,000 CFU）的完全有毒力的鼠疫耶尔森菌231菌株进行皮下攻击，该剂量比EV NIIEG本身能抵御的剂量高6-10倍。
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