利用拜氏梭菌从商业底物液化玉米淀粉中生产丙酮丁醇（AB），并通过气提进行产物回收。
Production of acetone butanol (AB) from liquefied corn starch, a commercial substrate, using Clostridium beijerinckii coupled with product recovery by gas stripping.
作者信息
Ezeji Thaddeus C, Qureshi Nasib, Blaschek Hans P
机构信息
Biotechnology and Bioengineering Group, Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801, USA.
出版信息
J Ind Microbiol Biotechnol. 2007 Dec;34(12):771-7. doi: 10.1007/s10295-007-0253-1. Epub 2007 Oct 10.
A potential industrial substrate (liquefied corn starch; LCS) has been employed for successful acetone butanol ethanol (ABE) production. Fermentation of LCS (60 g l(-1)) in a batch process resulted in the production of 18.4 g l(-1) ABE, comparable to glucose: yeast extract based medium (control experiment, 18.6 g l(-1) ABE). A batch fermentation of LCS integrated with product recovery resulted in 92% utilization of sugars present in the feed. When ABE was recovered by gas stripping (to relieve inhibition) from the fed-batch reactor fed with saccharified liquefied cornstarch (SLCS), 81.3 g l(-1) ABE was produced compared to 18.6 g l(-1) (control). In this integrated system, 225.8 g l(-1) SLCS sugar (487 % of control) was consumed. In the absence of product removal, it is not possible for C. beijerinckii BA101 to utilize more than 46 g l(-1) glucose. A combination of fermentation of this novel substrate (LCS) to butanol together with product recovery by gas stripping may economically benefit this fermentation.
一种潜在的工业底物（液化玉米淀粉；LCS）已被用于成功生产丙酮丁醇乙醇（ABE）。以分批发酵工艺发酵60 g l⁻¹ 的LCS，产生了18.4 g l⁻¹ 的ABE，与基于葡萄糖：酵母提取物的培养基（对照实验，18.6 g l⁻¹ 的ABE）相当。将LCS的分批发酵与产物回收相结合，可使进料中存在的糖利用率达到92%。当通过气提从进料分批反应器中回收ABE（以减轻抑制作用）时，进料为糖化液化玉米淀粉（SLCS），产生了81.3 g l⁻¹ 的ABE，而对照为18.6 g l⁻¹。在这个集成系统中，消耗了225.8 g l⁻¹ 的SLCS糖（对照的487%）。在没有产物去除的情况下，拜氏梭菌BA101无法利用超过46 g l⁻¹ 的葡萄糖。将这种新型底物（LCS）发酵生产丁醇与通过气提回收产物相结合，可能会给这种发酵带来经济效益。
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