Liu Xia, Wu Zhan, Nie Huagui, Liu Ziling, He Yan, Yeung E S
State Key Laboratory of Chemo/Biosensing and Chemometrics, BioMedical Engineering Center, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, PR China.
Anal Chim Acta. 2007 Oct 29;602(2):229-35. doi: 10.1016/j.aca.2007.09.020. Epub 2007 Sep 19.
We examined the adsorption of single YOYO-1-labeled lambda-DNA molecules at glass surfaces after treatment with various chemical cleaning methods by using total internal reflection fluorescence microscopy (TIRFM). The characteristics of these surfaces were further assessed using contact angle (CA) measurements and atomic force microscopy (AFM). By recording the real-time dynamic motion of DNA molecules at the liquid/solid interface, subtle differences in adsorption affinities were revealed. The results indicate that the driving force for adsorption of DNA molecules on glass surfaces is mainly hydrophobic interaction. We also found that surface topography plays a role in the adsorption dynamics.
我们使用全内反射荧光显微镜（TIRFM）研究了经各种化学清洗方法处理后的玻璃表面上单个YOYO-1标记的λ-DNA分子的吸附情况。通过接触角（CA）测量和原子力显微镜（AFM）进一步评估了这些表面的特性。通过记录DNA分子在液/固界面的实时动态运动，揭示了吸附亲和力的细微差异。结果表明，DNA分子在玻璃表面吸附的驱动力主要是疏水相互作用。我们还发现表面形貌在吸附动力学中起作用。
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