基于由纳米结构聚苯胺胶体悬浮液衍生的电泳沉积导电聚合物薄膜的胆固醇生物传感器。
Cholesterol biosensor based on electrophoretically deposited conducting polymer film derived from nano-structured polyaniline colloidal suspension.
作者信息
Dhand Chetna, Singh S P, Arya Sunil K, Datta Monika, Malhotra B D
机构信息
Biomolecular Electronics and Conducting Polymer Research Group, National Physical Laboratory, Dr. K.S. Krishnan Marg, New Delhi 110012, India.
出版信息
Anal Chim Acta. 2007 Oct 29;602(2):244-51. doi: 10.1016/j.aca.2007.09.028. Epub 2007 Sep 21.
Cholesterol oxidase (ChOx) has been covalently immobilized onto electrophoretically deposited conducting polymer film (on indium-tin-oxide (ITO) glass plate) derived from nano-structured polyaniline (PANI) colloidal suspension using N-ethyl-N'-(3-dimethylaminopropyl) corbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry. These PANI/ITO and ChOx/PANI/ITO electrodes have been characterized using ultraviolet-visible (UV-vis), Fourier transform-infrared (FT-IR), scanning electron microscopy (SEM), and impedance spectroscopy techniques, respectively. These ChOx/PANI/ITO bio-electrodes exhibit linearity from 25 to 400 mg dL(-1) of cholesterol, detection limit as 25 mg dL(-1) and sensitivity as 7.76x10(-5) Abs(mg/dL)(-1). The value of the apparent Michaelis-Menten constant (Km(app)) calculated from amperometric response studies has been found to be 0.62 mM for ChOx/PANI/ITO bio-electrode.
胆固醇氧化酶（ChOx）已通过N-乙基-N'-(3-二甲氨基丙基)碳二亚胺（EDC）和N-羟基琥珀酰亚胺（NHS）化学方法共价固定在由纳米结构聚苯胺（PANI）胶体悬浮液衍生的电泳沉积导电聚合物膜（在氧化铟锡（ITO）玻璃板上）上。这些聚苯胺/ITO电极和胆固醇氧化酶/聚苯胺/ITO电极分别使用紫外可见（UV-vis）、傅里叶变换红外（FT-IR）、扫描电子显微镜（SEM）和阻抗谱技术进行了表征。这些胆固醇氧化酶/聚苯胺/ITO生物电极在25至400 mg dL⁻¹的胆固醇浓度范围内呈线性，检测限为25 mg dL⁻¹，灵敏度为7.76×10⁻⁵ Abs(mg/dL)⁻¹。通过安培响应研究计算得出的胆固醇氧化酶/聚苯胺/ITO生物电极的表观米氏常数（Km(app)）值为0.62 mM。
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