利用肽-主要组织相容性复合体四聚体技术研究金黄色葡萄球菌蛋白与人类细胞之间的相互作用。
Use of peptide-major histocompatibility complex tetramer technology to study interactions between Staphylococcus aureus proteins and human cells.
作者信息
Massey Ruth C, Scriba Thomas J, Brown Eric L, Phillips Rodney E, Sewell Andrew K
机构信息
Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, United Kingdom.
出版信息
Infect Immun. 2007 Dec;75(12):5711-5. doi: 10.1128/IAI.00875-07. Epub 2007 Oct 15.
Abstract
In this study, we report the use of peptide-major histocompatibility complex tetramer technology to study the interactions that occur between Staphylococcus aureus proteins and human leukocytes. We demonstrated that this technology can be used to study the activity of superantigens such as toxic shock syndrome toxin 1 and also found that despite similarities to known proteins (i.e., major histocompatibility complex [MHC] class II molecules and superantigens), the S. aureus Eap protein does not block MHC-T-cell receptor interactions and is not a superantigen. Instead, it has nonspecific cross-linking activity that is dependent upon having at least two of its six 110-amino-acid repeats.
摘要
在本研究中，我们报告了使用肽-主要组织相容性复合体四聚体技术来研究金黄色葡萄球菌蛋白与人类白细胞之间发生的相互作用。我们证明了该技术可用于研究诸如中毒性休克综合征毒素1等超抗原的活性，并且还发现，尽管金黄色葡萄球菌Eap蛋白与已知蛋白（即主要组织相容性复合体[MHC] II类分子和超抗原）存在相似性，但它并不阻断MHC- T细胞受体相互作用，也不是超抗原。相反，它具有非特异性交联活性，这种活性依赖于其六个110个氨基酸的重复序列中至少有两个。
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