[Construction of 3D complex of porous beta-tricalcium phosphate/collagen scaffolds and dog periodontal ligament cells].

OBJECTIVE

To investigate the construction of 3D complex of porous beta-tricalcium phosphate/collagen scaffolds (beta-TCP/col) and dog periodontal ligament cells (PDLCs).

METHODS

Dog PDLCs were isolated, cultured and identified. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to evaluate the cytotoxicity of beta-TCP/col on the proliferation of PDLCs. The cells were seeded onto porous beta-TCP/col scaffolds. The cellular capability of adhesion and growth on porous beta-TCP/col surface was investigated visually by scanning electron microscopy (SEM).

RESULTS

The cytotoxicity assay indicated that there was no significant difference between beta-TCP/col and the control during the 7 days (P>0.05). SEM showed cells successfully adhered to porous beta-TCP/col scaffolds and spread extensively. Matrix secretions were found on the cell surface.

CONCLUSION

Porous beta-tricalcium phosphate/collagen scaffolds were of good biocompatibility to the dog periodontal ligament cells, and were potential ideal candidates for periodontal tissue engineering.