用于优化PCR设计的物理原理和可视化OMP软件。

Physical principles and visual-OMP software for optimal PCR design.

作者信息

SantaLucia John

机构信息

Department of Chemistry, Wayne State University, Detroit, MI, USA.

出版信息

Methods Mol Biol. 2007;402:3-34. doi: 10.1007/978-1-59745-528-2_1.

DOI:10.1007/978-1-59745-528-2_1

PMID:17951788

Abstract

The physical principles of DNA hybridization and folding are described within the context of how they are important for designing optimal PCRs. The multi-state equilibrium model for computing the concentrations of competing unimolecular and bimolecular species is described. Seven PCR design "myths" are stated explicitly, and alternative proper physical models for PCR design are described. This chapter provides both a theoretical framework for understanding PCR design and practical guidelines for users. The Visual-OMP (oligonucleotide modeling platform) package from DNA Software, Inc. is also described.

摘要

DNA杂交和折叠的物理原理在其对设计最佳聚合酶链式反应（PCR）的重要性的背景下进行了描述。描述了用于计算竞争性单分子和双分子物种浓度的多状态平衡模型。明确阐述了七个PCR设计“误区”，并描述了用于PCR设计的替代合适物理模型。本章既提供了理解PCR设计的理论框架，也为用户提供了实用指南。还介绍了DNA软件公司的Visual-OMP（寡核苷酸建模平台）软件包。