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持续使用“错误的”细胞系。

Persistent use of "false" cell lines.

作者信息

Lacroix Marc

机构信息

InTextoResearch, Baelen, Wallonia, Belgium.

出版信息

Int J Cancer. 2008 Jan 1;122(1):1-4. doi: 10.1002/ijc.23233.

Abstract

From HeLa and its multiple identities, to MDA-MB-435, erroneously and widely used as breast cancer cells, the history of cancer cell lines is rich in misidentification and cross-contamination events. Despite the fact that these problems were regularly signaled during the last decades, many actors of research still seem to ignore them. A never-ending story? Solutions exist, notably based on recent technical advances in cell line authentication (short tandem repeat analysis). However, a collaborative action involving users of cell lines, cell banks, journals and funding agencies is needed to achieve success.

摘要

从海拉细胞及其多重身份,到被错误地广泛用作乳腺癌细胞的MDA-MB-435,癌细胞系的历史充斥着错误鉴定和交叉污染事件。尽管在过去几十年里这些问题经常被指出,但许多研究人员似乎仍然忽视它们。这是一个永无止境的故事吗?解决方案是存在的,特别是基于细胞系鉴定(短串联重复序列分析)的最新技术进展。然而,需要细胞系使用者、细胞库、期刊和资助机构共同采取行动才能取得成功。

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