Pinart M, Serrano-Mollar A, Negri E M, Cabrera R, Rocco P R M, Romero P V
Laboratory of Experimental Pneumology, IDIBELL, L'Hospitalet, Barcelona, Spain.
Respir Physiol Neurobiol. 2008 Feb 1;160(2):196-203. doi: 10.1016/j.resp.2007.09.012. Epub 2007 Sep 26.
The impact of lung remodelling in respiratory mechanics has been widely studied in bleomycin-induced lung injury. However, little is known regarding the relationship between the amount of lung inflammation and pulmonary tissue mechanics. For this purpose, rats were intratracheally instilled with bleomycin (n=29) or saline (n=8) and sacrificed at 3, 7, or 15 days. Forced oscillatory mechanics as well as indices of remodelling (elastic fibre content and hydroxyproline) and inflammation (myeloperoxidase content, total cell count, alveolar wall thickness, and lung water content) were studied in lung tissue strips. Tissue resistance increased significantly at day 15, while hysteresivity was significantly higher in bleomycin group compared to control at all time points. Elastic fibres, hydroxyproline and myeloperoxidase contents augmented after bleomycin at days 7 and 15. Tissue resistance and hysteresivity were significantly correlated with myeloperoxidase, elastic fibre and lung water content. In conclusion, inflammatory structural changes and elastogenesis are the main determinants for hysteretic changes in this 2-week bleomycin-induced lung injury model.
在博来霉素诱导的肺损伤中，肺重塑对呼吸力学的影响已得到广泛研究。然而，关于肺部炎症程度与肺组织力学之间的关系，人们所知甚少。为此，给大鼠气管内注入博来霉素（n = 29）或生理盐水（n = 8），并在3天、7天或15天时处死。对肺组织条进行强迫振荡力学以及重塑指标（弹性纤维含量和羟脯氨酸）和炎症指标（髓过氧化物酶含量、总细胞计数、肺泡壁厚度和肺含水量）的研究。在第15天时组织阻力显著增加，而在所有时间点，博来霉素组的滞后现象均显著高于对照组。在第7天和第15天，博来霉素处理后弹性纤维、羟脯氨酸和髓过氧化物酶含量增加。组织阻力和滞后现象与髓过氧化物酶、弹性纤维和肺含水量显著相关。总之，在这个为期两周的博来霉素诱导的肺损伤模型中，炎症结构变化和弹性生成是滞后变化的主要决定因素。
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