羊水过少诱导的兔胎儿肺发育不全中II型肺细胞的超微结构评估
Ultrastructural evaluation of type II pneumocytes in the hypoplastic lung of rabbit fetuses induced by oligohydramnios.
作者信息
Asabe Koushi, Oka Yoichiro, Kai Hiroki, Shirakusa Takayuki
机构信息
Division of Pediatric Surgery, Maternity and Perinatal Care Center, Fukuoka University Hospital, Jonan-ku, Fukuoka, Japan.
出版信息
J Obstet Gynaecol Res. 2007 Dec;33(6):777-80. doi: 10.1111/j.1447-0756.2007.00655.x.
AIM
The present study was carried out using electron microscopy to evaluate the expression of lamellar bodies in type II pneumocytes of fetal rabbit lungs with hypoplasia induced by oligohydramnios.
METHODS
Using an amniotic shunting rabbit model, the lungs obtained from 32 fetuses, including 16 experimental fetuses (shunting group) and 16 control fetuses (control group), were used for electron microscopic studies. At least 250 type II pneumocytes per fetus were photographed. The number of lamellar bodies per type II pneumocyte was counted.
RESULTS
The number of lamellar bodies per type II pneumocyte of the shunting group was consistently and significantly less than that of the control group (5.49 +/- 2.07 vs 7.58 +/- 2.08; P < 0.01).
CONCLUSIONS
These results suggest that the occurrence of hypoplastic lungs induced by oligohydramnios is associated with an immaturity of the surfactant system, especially the expression of the lamellar bodies of type II pneumocytes.
目的
本研究采用电子显微镜评估羊水过少诱导的肺发育不全胎兔肺Ⅱ型肺细胞中板层小体的表达。
方法
利用羊膜分流兔模型，从32只胎儿获取的肺用于电子显微镜研究，其中包括16只实验胎儿（分流组）和16只对照胎儿（对照组）。每个胎儿至少拍摄250个Ⅱ型肺细胞。计算每个Ⅱ型肺细胞的板层小体数量。
结果
分流组每个Ⅱ型肺细胞的板层小体数量始终显著少于对照组（5.49±2.07对7.58±2.08；P<0.01）。
结论
这些结果表明，羊水过少诱导的肺发育不全的发生与表面活性物质系统的不成熟有关，尤其是Ⅱ型肺细胞板层小体的表达。
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