细胞外基质、整合素和细胞骨架在离心负荷机械转导中的作用。
The role of extracellular matrix, integrins, and cytoskeleton in mechanotransduction of centrifugal loading.
作者信息
Li Juan, Zhao Zhihe, Wang Jun, Chen Guoping, Yang Jingyuan, Luo Songjiao
机构信息
Department of Orthodontics, West China College of Stomatology, Sichuan University, 14#, 3rd section, Renmin South Road, Chengdu 610041, PR China.
出版信息
Mol Cell Biochem. 2008 Feb;309(1-2):41-8. doi: 10.1007/s11010-007-9641-0. Epub 2007 Nov 16.
The study was aimed to investigate the role of the "extracellular matrix (ECM)-integrins-cytoskeleton" signal pathway in mechanotransduction of centrifugal loading. MG-63 osteoblasts were exposed to centrifugal loading at 209xg for 10 min. Uncentrifuged cells and centrifuged cells that have been trypsinized and suspended in liquors were designed as control. The changes in F-actin and alpha-actin cytoskeleton, gene transcription of ECM components, and integrins expression were analyzed by LSCM, Real-Time RT-PCR and FCM, respectively. A temporary and fast reversible change was observed in F-actin and alpha-actin cytoskeleton. And the change was paralleled with the fast autoregulation in gene transcription of ECM components of fibronection, osteopontin and Collagen I, and integrins expression of both alpha2 and beta1 subunits. The result suggested that cytoskeleton was a possible mechanical sensor to centrifugal stimuli, and the cytoskeleton regulation to centrifugal loading was in an ECM-dependent and integrin-mediated manner.
本研究旨在探讨“细胞外基质（ECM）-整合素-细胞骨架”信号通路在离心加载机械转导中的作用。将MG-63成骨细胞暴露于209xg的离心加载下10分钟。未离心的细胞以及经胰蛋白酶消化并悬浮于液体中的离心细胞被设计为对照。分别通过激光扫描共聚焦显微镜（LSCM）、实时逆转录聚合酶链反应（Real-Time RT-PCR）和流式细胞术（FCM）分析F-肌动蛋白和α-肌动蛋白细胞骨架的变化、ECM成分的基因转录以及整合素的表达。在F-肌动蛋白和α-肌动蛋白细胞骨架中观察到了一种短暂且快速可逆的变化。并且这种变化与纤连蛋白、骨桥蛋白和I型胶原的ECM成分基因转录以及α2和β1亚基的整合素表达的快速自动调节平行。结果表明，细胞骨架可能是离心刺激的一种机械传感器，并且细胞骨架对离心加载的调节是以ECM依赖和整合素介导的方式进行的。
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