人类端粒G-四链体形成T环

T-loop formation by human telomeric G-quadruplex.

作者信息

Xu Yan, Sato Hiroyuki, Shinohara Ken-Ichi, Komiyama Makoto, Sugiyama Hiroshi

机构信息

Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan.

出版信息

Nucleic Acids Symp Ser (Oxf). 2007(51):243-4. doi: 10.1093/nass/nrm122.

DOI:10.1093/nass/nrm122

PMID:18029677

Abstract

T-loops were a proposed telomere structure and have been speculated to protect telomeres and regulate telomerase. In the current study, we examined the structural and thermodynamic properties of the dimeric G-quadruplex of human telomeric DNA using chemical modifications and CD spectroscopy. We demonstrated that the dimeric G-quadruplex topology induced the T-loop formation in K(+) solution.

摘要

T环是一种提出的端粒结构，据推测它可保护端粒并调节端粒酶。在本研究中，我们使用化学修饰和圆二色光谱法研究了人类端粒DNA二聚体G-四链体的结构和热力学性质。我们证明，二聚体G-四链体拓扑结构在钾离子溶液中诱导了T环的形成。