糖构象在二核苷酸水平上控制（6-4）光产物的形成。

The sugar conformation governs (6-4) photoproduct formation at the dinucleotide level.

作者信息

Desnous Céline, Babu B Ravindra, Moriou Céline, Mayo Javier Ulises Ortiz, Favre Alain, Wengel Jesper, Clivio Pascale

机构信息

Institut de Chimie des Substances Naturelles, CNRS, 1 Avenue de la Terrasse, 91190 Gif sur Yvette, France, Nucleic Acid Center, Department of Physics and Chemistry, University of Southern Denmark, 5230 Odense M, Denmark.

出版信息

J Am Chem Soc. 2008 Jan 9;130(1):30-1. doi: 10.1021/ja077095q. Epub 2007 Dec 11.

DOI:10.1021/ja077095q

PMID:18069836

Abstract

The LNA dinucleotide mimic of TpT whose two-sugar puckers are locked in the C3'-endo conformation selectively produces the corresponding cyclobutane pyrimidine dimer under 254 nm irradiation. In the natural series (TpT) the sugar puckers are in a major C2'-endo sugar conformation and the (6-4) photoproduct is also produced. Consequently, this study demonstrates that the C2'-endo conformation of the sugar pucker is necessary for (6-4) photoproduct formation.

摘要

两个糖环构象锁定在C3'-内型构象的TpT的LNA二核苷酸类似物在254nm照射下选择性地产生相应的环丁烷嘧啶二聚体。在天然序列（TpT）中，糖环构象主要为C2'-内型，并且也会产生（6-4）光产物。因此，本研究表明糖环的C2'-内型构象对于（6-4）光产物的形成是必要的。