铽敏化荧光结合二阶散射法测定乌利沙星及其应用

Determination of ulifloxacin by terbium-sensitized fluorescence with second-order scattering and its applications.

作者信息

Wang Xiaoli, Shen Bo, Zhao Huichun, Jin Linpei

机构信息

College of Chemistry, Beijing Normal University, Beijing 100875, P. R. China.

出版信息

Anal Sci. 2007 Dec;23(12):1373-6. doi: 10.2116/analsci.23.1373.

DOI:10.2116/analsci.23.1373

PMID:18071221

Abstract

This paper reports the determination of ulifloxacin (UFX) by terbium-sensitized fluorescence using a second-order scattering method. UFX and Tb(III) ion form a fluorescence complex in aqueous solution, and its maximum excitation and emission wavelengths are located at 273 and 545 nm, respectively. In optimum conditions, the relative intensity at 545 nm has a linear relationship to the concentration of UFX in the range of 2.0 x 10(-8) - 1.0 x 10(-5) mol L(-1) and the detection limit is 3.9 x 10(-9) mol L(-1). The proposed method was applied to the determination of UFX in spiked human serum and urine satisfactorily. The luminescence property of UFX is also discussed by comparing with norfloxacin (NFLX) and ofloxacin (OFLX).

摘要

本文报道了采用二阶散射法，利用铽敏化荧光法测定乌利沙星（UFX）。UFX与Tb(III)离子在水溶液中形成荧光络合物，其最大激发波长和发射波长分别位于273nm和545nm。在最佳条件下，545nm处的相对强度与UFX浓度在2.0×10(-8) - 1.0×10(-5) mol L(-1)范围内呈线性关系，检测限为3.9×10(-9) mol L(-1)。该方法用于加标人血清和尿液中UFX的测定，结果令人满意。通过与诺氟沙星（NFLX）和氧氟沙星（OFLX）比较，还讨论了UFX的发光性质。