Ostan Ipek, Saglam Hüsniye, Limoncu M Emin, Ertabaklar Hatice, Toz Seray Ozensoy, Ozbel Yusuf, Ozbilgin Ahmet
Vocational School of Health Services, Celal Bayar University, Manisa, Turkey.
New Microbiol. 2007 Oct;30(4):439-45.
This study aimed to evaluate the in vitro and in vivo leishmanicidal activity of an endemic Turkish plant and compare its efficacy with a reference drug. In addition to the in vitro activities of the ethanol, acidified and alkaloid extracts and furoquinoline alkaloids skimmianine and gamma-fagarine, in vivo antileishmanial activitiy of the acidified extract of Haplophyllum myrtifolium Boiss. (Rutaceae) were investigated against Leishmania tropica (L. tropica), a causative agent of cutaneous leishmaniasis. All the extracts and pure compounds showed in vitro inhibitory activity against the promastigotes of. L. tropica. The in vitro 50% inhibitory concentrations of y-fagarine, acidified extract, ethanol extract, skimmianine and alkaloid extract against promastigotes were determined as 8.7, 9.4, 10.9, 25.7 and 25.8 microg/ml respectively. In vivo results of Haplophyllum myrtifolium acidified extract showed that this plant has a limited effect on decreasing the lesion size of experimental mice infected with Leishmania tropica. To the best of our knowledge, this is the first time both the in vitro and in vivo antileishmanial activity of Haplophyllum mrytifolium have been reported in the same research.
本研究旨在评估一种土耳其本土植物的体外和体内抗利什曼原虫活性，并将其疗效与一种参考药物进行比较。除了乙醇提取物、酸化提取物、生物碱提取物以及呋喃喹啉生物碱斯氏碱和γ-法加林的体外活性外，还研究了桃叶叶芸香（芸香科）酸化提取物对皮肤利什曼病病原体热带利什曼原虫的体内抗利什曼原虫活性。所有提取物和纯化合物均对热带利什曼原虫前鞭毛体表现出体外抑制活性。γ-法加林、酸化提取物、乙醇提取物、斯氏碱和生物碱提取物对前鞭毛体的体外50%抑制浓度分别测定为8.7、9.4、10.9、25.7和25.8微克/毫升。桃叶叶芸香酸化提取物的体内结果表明，该植物对减小感染热带利什曼原虫的实验小鼠的病变大小作用有限。据我们所知，这是首次在同一研究中报道桃叶叶芸香的体外和体内抗利什曼原虫活性。
