In vitro expression of belladonna mottle virus genome.
作者信息
Jacob A N, Savithri H S
机构信息
Department of Biochemistry, Indian Institute of Science, Bangalore.
出版信息
Indian J Biochem Biophys. 1991 Oct-Dec;28(5-6):456-60.
PMID:1812082
Abstract
In vitro translation of belladonna mottle virus BDMV(I) genomic RNA in a rabbit reticulocyte lysate system produced proteins of Mr 210,000, 150,000 and 78,000 which form the non-structural proteins. The coat protein, on the other hand, was expressed from a subgenomic RNA which was found to be encapsidated in the empty capsids forming the top component viral particles. The implications of subgenomic RNA encapsidation in viral replication and assembly are discussed.
摘要
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