颈动脉斑块中金属蛋白酶的表达及其与颈动脉内膜剥脱术前、后血浆水平的相关性。

Metalloproteinase expression in carotid plaque and its correlation with plasma levels before and after carotid endarterectomy.

作者信息

Taurino Maurizio, Raffa Salvatore, Mastroddi Massimo, Visco Vincenzo, Rizzo Luigi, Torrisi Maria Rosaria, Faraglia Vittorio

机构信息

U.O.C. Chirurgia Vascolare, S. Andrea Hospital, 2nd Medical School, University of Rome La Sapienza, Rome, Italy.

出版信息

Vasc Endovascular Surg. 2007;41(6):516-21. doi: 10.1177/1538574407307405.

DOI:10.1177/1538574407307405

PMID:18166633

Abstract

PURPOSE

To assess baseline and follow-up plasma concentrations of metalloproteinase-9 (MMP-9), MMP-2, and tissue inhibitor of metalloproteinase-2 (TIMP-2) in patients undergoing carotid thromboendarterectomy (TEA) in relation to tissue expression and diagnostic features.

BASIC METHODS

Using sandwich enzyme-linked immunosorbent assay, plasma levels of enzymes were determined in 15 patients undergoing carotid TEA. Tissue sections were incubated with specific antibodies and fluorescence intensity was analyzed.

PRINCIPAL FINDINGS

MMP-9 levels were higher in patients with carotid stenosis versus controls, significantly in those with cerebral lesions at neuroimaging. MMP-9 levels decreased in 93.4% of the patients at 1 month. MMP-2 levels tended to increase 30 days after surgery. TIMP-2 showed no difference.

CONCLUSIONS

High concentrations of MMP-9 found in patients with carotid stenosis and cerebral lesions suggest that MMP-9 assay could be useful in the evaluation of all carotid lesions to help identify those at highest risk of a neurologic event.

摘要