Determination of 9-[(2-phosphonylmethoxy)ethyl]adenine in rat urine by high-performance liquid chromatography with fluorescence detection.

作者信息

Russell J W, Marrero D, Whiterock V J, Klunk L J, Starrett J E

机构信息

Department of Metabolism and Pharmacokinetics, Bristol-Myers Squibb Company, Wallingford, CT 06492.

出版信息

J Chromatogr. 1991 Dec 6;572(1-2):321-6. doi: 10.1016/0378-4347(91)80498-2.

A high-performance liquid chromatographic (HPLC) method for the determination of 9-[(2-phosphonylmethoxy)ethyl]adenine (PMEA) in urine is described. The procedure includes treatment of the urine sample with chloroacetaldehyde to form the fluorescent 1,N6-ethenoadenosine derivative, which was analyzed by reversed-phase HPLC with fluorometric detection. Validation of the method showed good sensitivity, precision and reproducibility. The method is useful for the study of urinary excretion of PMEA in the rat.