Paf1复合物在RNA聚合酶II快速诱导转录时促进组蛋白的置换。
The Paf1 complex promotes displacement of histones upon rapid induction of transcription by RNA polymerase II.
作者信息
Marton Heather A, Desiderio Stephen
机构信息
Department of Molecular Biology and Genetics and Program in Immunology, Institute for Cell Engineering, The Johns Hopkins University School of Medicine, 733 North Broadway, Baltimore, Maryland 21205, USA.
出版信息
BMC Mol Biol. 2008 Jan 14;9:4. doi: 10.1186/1471-2199-9-4.
BACKGROUND
The yeast Paf1 protein complex is required for efficient transcription elongation by RNA polymerase II (RNA pol II), but the precise role of the complex has been unclear.
RESULTS
Here we show that depletion of the Ctr9 or Paf1 component of the Paf1 complex delays the loss of histones from the GAL1 gene upon induction. This delay in histone removal is accompanied by a decrease in association of RNA pol II with GAL1 and altered distribution of the polymerase along the locus.
CONCLUSION
These observations may explain why initial induction of GAL transcripts is reduced in Ctr9- or Paf1-deficient cells, and is consistent with a model suggesting that the Paf1 complex and the histone modifications that it mediates increase efficiency of transcriptional elongation by promoting nucleosomal destabilization and histone removal.
背景
酵母Paf1蛋白复合物是RNA聚合酶II（RNA pol II）高效转录延伸所必需的，但该复合物的确切作用尚不清楚。
结果
我们在此表明，Paf1复合物的Ctr9或Paf1组分的缺失会延迟诱导后GAL1基因上组蛋白的丢失。组蛋白去除的这种延迟伴随着RNA pol II与GAL1的结合减少以及聚合酶沿该基因座分布的改变。
结论
这些观察结果可能解释了为什么在缺乏Ctr9或Paf1的细胞中GAL转录本的初始诱导会减少，并且与一种模型一致，该模型表明Paf1复合物及其介导的组蛋白修饰通过促进核小体去稳定化和组蛋白去除来提高转录延伸效率。
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