通过液相色谱-质谱联用技术鉴定和定量海洋腹足纲动物织纹螺中的河豚毒素

Identification and quantification of tetrodotoxin in the marine gastropod Nassarius by LC-MS.

作者信息

Huang Hong-Nan, Lin Jie, Lin Hong-Lin

机构信息

Department of Physical and Chemical Analysis, Fujian Center for Disease Control and Prevention, Fuzhou 350001, PR China; School of Pharmacy, Fujian Medical University, Fuzhou 350004, PR China.

出版信息

Toxicon. 2008 Apr;51(5):774-9. doi: 10.1016/j.toxicon.2007.12.003. Epub 2007 Dec 14.

DOI:10.1016/j.toxicon.2007.12.003

PMID:18222512

Abstract

Tetrodotoxin (TTX) has been usually analyzed in marine and other sources by HPLC separation, followed by alkaline treatment and fluorescence assay. However, the applications of such methods were limited because of their tedious extraction and derivation procedures. A simpler, rapid LC-MS method, using selected ion-monitoring, has now been developed, and used to identify and quantitate TTX in a marine gastropod of the genus Nassarius, collected from Fijian province of China. The toxin was confirmed comparable to authentic TTX by MS and MSn. In addition, a moderate amount of TTX was detected in the Nassarius by this novel approach, suggesting that Nassarius would be a potential food poison source.

摘要

河豚毒素（TTX）通常通过高效液相色谱（HPLC）分离，随后进行碱性处理和荧光测定，来分析海洋及其他来源中的河豚毒素。然而，由于这些方法的提取和衍生程序繁琐，其应用受到限制。现在已经开发出一种更简单、快速的液相色谱-质谱（LC-MS）方法，采用选择离子监测，用于鉴定和定量从中国福建省采集的织纹螺属海洋腹足动物中的TTX。通过质谱（MS）和串联质谱（MSn）证实该毒素与标准TTX相当。此外，通过这种新方法在织纹螺中检测到了适量的TTX，这表明织纹螺可能是一种潜在的食物中毒源。