利用吸收光谱和荧光光谱对DNA和RNA进行定量分析。

Quantification of DNA and RNA with absorption and fluorescence spectroscopy.

作者信息

Gallagher S R

机构信息

Motorola Corporation, Tempe, Arizona, USA.

出版信息

Curr Protoc Cell Biol. 2001 May;Appendix 3:Appendix 3D. doi: 10.1002/0471143030.cba03ds07.

DOI:10.1002/0471143030.cba03ds07

PMID:18228286

Abstract

Reliable quantitation of nanogram and microgram amounts of DNA and RNA in solution is essential to researchers in cell and molecular biology. In addition to the traditional absorbance measurements at 260 nm, two more sensitive fluorescence techniques-Hoescht 33258 dye binding, ethidium bromide binding are described. These three procedures cover a range from 5 to 10 ng/ml DNA to 50 mg/ml DNA.

摘要

对细胞与分子生物学领域的研究人员来说，可靠地定量分析溶液中纳克和微克级别的DNA和RNA至关重要。除了传统的260nm吸光度测量方法外，还介绍了另外两种更灵敏的荧光技术——Hoechst 33258染料结合法和溴化乙锭结合法。这三种方法覆盖了从5至10 ng/ml DNA到50 mg/ml DNA的范围。