Poon Peter C, Gupta Dimpy, Shoichet Molly S, Tator Charles H
Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada.
Spine (Phila Pa 1976). 2007 Dec 1;32(25):2853-9. doi: 10.1097/BRS.0b013e31815b7e6b.
Experimental investigation of an acute thoracic spinal cord injury model in rats involving acute clip compression that simulates human injury.
To assess the dose-response of this model for the relationship between the force of injury on the rat thoracic spinal cord and histological and functional outcome measures.
Acute extradural clip compression injury has been a reliable model for producing acute experimental cervical spinal cord injury; however, this model has not been formally evaluated with dose-response curves for acute injury of the thoracic spinal cord.
After laminectomy at T2 in Sprague-Dawley rats, a modified aneurysm clip exerting a closing force of 20, 26, or 35 g was applied extradurally around the spinal cord at T2, and then rapidly released with cord compression persisting for 1 minute. These forces were selected to simulate acute compression injuries of mild to moderate, moderate, and moderate to severe degrees, respectively (n = 8/group). Motor activity was assessed weekly for 4 weeks with the Basso, Beattie, and Bresnahan (BBB) open field locomotor test. The injured spinal cord was then examined histologically including quantification of cavitation.
A significant main effect was observed for clip force and BBB score (F(2,20) = 5.42, P = 0.013). For 4 weeks after injury, the BBB scores for the 20 g and 35 g clip injury groups were significantly different (P < 0.05). The cavitation volume at 4 weeks was directly proportional to the severity of injury: the 20 g group had significantly smaller cavities than the 35 g group (P < 0.05), and the cavitation volume correlated with the BBB scores.
The rat thoracic cord clip compression model is a reproducible, clinically relevant spinal cord injury model. This is the first time that the force of clip compression injury in the rat thoracic cord has been correlated with both functional and histologic outcome measures.
对大鼠急性胸段脊髓损伤模型进行实验研究，该模型采用模拟人类损伤的急性夹闭压迫法。
评估该模型中大鼠胸段脊髓损伤力与组织学和功能结局指标之间的剂量反应关系。
急性硬膜外夹闭压迫损伤一直是产生急性实验性颈段脊髓损伤的可靠模型；然而，该模型尚未通过胸段脊髓急性损伤的剂量反应曲线进行正式评估。
在Sprague-Dawley大鼠的T2节段进行椎板切除术后，使用一种改良的动脉瘤夹在T2节段脊髓硬膜外施加20、26或35克的闭合力，然后迅速释放，脊髓压迫持续1分钟。选择这些力分别模拟轻度至中度、中度和中度至重度的急性压迫损伤（每组n = 8）。每周使用Basso、Beattie和Bresnahan（BBB）旷场运动测试评估运动活动，持续4周。然后对损伤的脊髓进行组织学检查，包括空洞形成的量化。
观察到夹力和BBB评分有显著的主效应（F(2,20) = 5.42，P = 0.013）。损伤后4周，20克和35克夹伤组的BBB评分有显著差异（P < 0.05）。4周时的空洞体积与损伤严重程度成正比：20克组的空洞明显小于35克组（P < 0.05），且空洞体积与BBB评分相关。
大鼠胸段脊髓夹闭压迫模型是一种可重复的、与临床相关的脊髓损伤模型。这是首次将大鼠胸段脊髓夹闭压迫损伤力与功能和组织学结局指标相关联。
Zotero 插件