Chaplin D D, Brownstein B H
Howard Hughes Medical Institute and Washington University School of Medicine, St. Louis, Missouri, USA.
Curr Protoc Mol Biol. 2001 May;Chapter 6:Unit6.9. doi: 10.1002/0471142727.mb0609s20.
This unit provides an introduction to the use of yeast artificial chromosome-bearing yeast clones (hereafter referred to as YAC clones) in genome analysis. It describes criteria for designing a polymerase chain reaction (PCR) assay to be used in screening a YAC core library and discusses the rationale for verification and characterization of YAC clones obtained from these core laboratories. Protocols for maintaining YAC clones, analyzing YAC insert structure, preparing YAC DNA, and subcloning YAC inserts into other vectors are presented elsewhere in this volume.
本单元介绍了在基因组分析中使用携带酵母人工染色体的酵母克隆（以下简称YAC克隆）。它描述了设计用于筛选YAC核心文库的聚合酶链反应（PCR）检测的标准，并讨论了从这些核心实验室获得的YAC克隆的验证和表征的基本原理。本卷其他地方介绍了保存YAC克隆、分析YAC插入结构、制备YAC DNA以及将YAC插入片段亚克隆到其他载体中的方法。
