小鼠种群管理。
Mouse colony management.
作者信息
Conner David A
机构信息
Harvard Medical School, Cambridge, Massachusetts, USA.
出版信息
Curr Protoc Mol Biol. 2002 Feb;Chapter 23:Unit 23.8. doi: 10.1002/0471142727.mb2308s57.
PMID:18265310
Abstract
The management of mouse colonies created by gene targeting is described in this unit. It defines guidelines necessary to establish the colony, and describes screening for germline transmission, marking the mice for easy and consistent identification, and genotyping DNA by PCR or Southern blotting analysis. It also reviews the software currently available for colony data management.
摘要
本单元介绍了通过基因靶向创建的小鼠群体的管理方法。它定义了建立群体所需的指导方针，并描述了种系传递筛选、标记小鼠以便轻松且一致地识别，以及通过聚合酶链反应（PCR）或Southern印迹分析对DNA进行基因分型。它还回顾了目前可用于群体数据管理的软件。
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