Hattori Takasumi, Honda Yuki, Kino Kuniki, Kirimura Kohtaro
Department of Applied Chemistry, School of Science and Engineering, Waseda University, Tokyo, Japan.
J Biosci Bioeng. 2008 Jan;105(1):55-7. doi: 10.1263/jbb.105.55.
Mycelia of citric acid-producing Aspergillus niger WU-2223L show cyanide-insensitive respiration catalyzed by alternative oxidase. In this study, the constitutive expression of alternative oxidase gene (aox1) even at the stage of single-cell conidium in A. niger WU-2223L was found using the visual expression analysis system of aox1 with green fluorescent protein under microscopy observation.
产柠檬酸黑曲霉WU - 2223L的菌丝体表现出由交替氧化酶催化的氰化物不敏感呼吸作用。在本研究中，利用带有绿色荧光蛋白的交替氧化酶基因（aox1）的可视化表达分析系统，在显微镜观察下发现aox1在黑曲霉WU - 2223L的单细胞分生孢子阶段即组成性表达。
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