Improvement of transformation efficiency by bioactive-beads-mediated gene transfer using DNA-lipofectin complex as entrapped genetic material.
作者信息
Murakawa Tomoko, Kajiyama Shin'ichiro, Ikeuchi Tomohiko, Kawakami Shigeki, Fukui Kiichi
机构信息
Sasebo National College of Technology, Sasebo, Nagasaki, Japan.
出版信息
J Biosci Bioeng. 2008 Jan;105(1):77-80. doi: 10.1263/jbb.105.77.
PMID:18295726
Abstract
The efficiency of bioactive-beads-mediated plant transformation was improved using DNA-lipofectin complex as the entrapped genetic material instead of naked DNA used in the conventional method. In the improved method, beads aggregated and formed clusters around the protoplasts resulting in a 4-fold higher transformation efficiency than that by the conventional method.
摘要
Zotero 插件