Zhu Hongying, White Ian M, Suter Jonathan D, Zourob Mohammed, Fan Xudong
Department of Biological Engineering, University of Missouri-Columbia, 240D, Bond Life Sciences Center, Columbia, MO 65211, USA.
Analyst. 2008 Mar;133(3):356-60. doi: 10.1039/b716834a. Epub 2008 Jan 8.
We have demonstrated sensitive label-free virus detection using the opto-fluidic ring resonator (OFRR) sensor. The OFRR is a novel sensing platform that integrates the microfluidics and photonic sensing technology with a low detection limit and small volume. In our experiment, filamentous bacteriophage M13 was used as a safe model system. Virus samples were flowed through the OFRR whose surface was coated with M13-specific antibodies. We studied the sensor performance by monitoring in real-time the virus and antibody interaction. It is shown that OFRR can detect M13 with high specificity and sensitivity. The detection limit is approximately 2.3 x 10(3) pfu mL(-1) and the detection dynamic range spanned seven orders of magnitude. Theoretical analysis was also carried out to confirm the experimental results. Our study will lead to development of novel OFRR-based, sensitive, rapid, and low-cost micro total analysis devices for virus detection.
我们已经展示了使用光流体环形谐振器（OFRR）传感器进行灵敏的无标记病毒检测。OFRR是一种新型传感平台，它将微流体和光子传感技术集成在一起，具有低检测限和小体积的特点。在我们的实验中，丝状噬菌体M13被用作安全的模型系统。病毒样本流经表面涂有M13特异性抗体的OFRR。我们通过实时监测病毒与抗体的相互作用来研究传感器性能。结果表明，OFRR能够以高特异性和灵敏度检测M13。检测限约为2.3×10³ 噬菌斑形成单位/毫升（pfu mL⁻¹），检测动态范围跨越七个数量级。还进行了理论分析以证实实验结果。我们的研究将推动基于新型OFRR的灵敏、快速且低成本的微型全分析病毒检测设备的开发。
