Huang Dong-Mei, Huang Guang-Yin, Xu Li-Jun, Zhang Ming-Min
Institute of Integrated Traditional and Western Medicine, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhongguo Zhong Yao Za Zhi. 2007 Oct;32(20):2152-5.
To observe the effect of Bushen Yiqi Hexue recipe on endometrial P21 and PCNA expression of embryo implantation dysfunctional mice.
The expression of P21 protein was analyzed by means of immunoblot and immunohistochemical method. The expression of PCNA protein was analyzed by means of immunohistochemical method.
The expression and disappearance of P21 and PCNA protein in endometrium of embryo implantation dysfunctional mice lagged behind in a temporal and cell specific manner. Bushenyiqihexue recipe could improve the expression of P21 and PCNA protein in a temporal and cell specific manner, and then improve the decidual reaction of endometrium of embryo implantation dysfunctional mice.
Improving the expression of P21 and PCNA protein in endometrium and eventually regulating endometrium decidual reaction is one of the mechanisms of improved embryo implantaion by Bushen Yiqi Hexue recipe.
观察补肾益气活血方对胚胎着床功能障碍小鼠子宫内膜P21和PCNA表达的影响。
采用免疫印迹法和免疫组织化学法分析P21蛋白的表达。采用免疫组织化学法分析PCNA蛋白的表达。
胚胎着床功能障碍小鼠子宫内膜中P21和PCNA蛋白的表达及消失在时间和细胞特异性上滞后。补肾益气活血方可在时间和细胞特异性上改善P21和PCNA蛋白的表达，进而改善胚胎着床功能障碍小鼠子宫内膜的蜕膜反应。
提高子宫内膜中P21和PCNA蛋白的表达并最终调节子宫内膜蜕膜反应是补肾益气活血方改善胚胎着床的机制之一。
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