Larregle Ethel V, Varas Silvia M, Oliveros Liliana B, Martinez Luis D, Antón Rosa, Marchevsky Eduardo, Giménez María S
Department of Biochemistry and Biological Sciences, Faculty of Chemistry, Biochemistry and Pharmacy, National University of San Luis, IMBIO-SL, CONICET, San Luis, Argentina.
Food Chem Toxicol. 2008 May;46(5):1786-92. doi: 10.1016/j.fct.2008.01.018. Epub 2008 Jan 18.
We investigated the effect of exposition to cadmium (Cd, 15ppm for 8 weeks) through drinking water on liver lipid metabolism in adult male Wistar rats. As compared to metal non-exposed (control) rats, the serum triglycerides, cholesterol and LDL+VLDL cholesterol concentrations increased. This was associated to a decrease of lipoprotein lipase activity in post heparinic plasma. The VLDL secretion from liver was not modified. Cd treatment increased triglycerides and decreased esterified cholesterol contents in liver. The high triglyceride mass was related to the increased glycerol-3-phosphate acyltransferase mRNA expression. In addition, the liver fatty acids synthesis increased, as determined by an increment of fatty acid synthetase and isocitrate dehydrogenase activities, and [(14)C]-acetate incorporation into saponifiable lipid fraction. The relative percentage of palmitic acid (16:0) and total saturated fatty acids were increased compared with control. Hepatic glucose-6-phosphate dehydrogenase, malic dehydrogenase and cholesteryl ester hydrolase activities were unchanged. In liver, the Cd treatment decreased triglyceride and cholesterol in mitochondria, also increased triglyceride in cytosol, and cholesterol and phospholipid contents in nuclei, compared with control. In addition, an increase of nuclei phosphatidylcholine synthesis was observed. Cd exposure alters directly or indirectly the serum lipid content and liver lipid metabolism.
我们研究了成年雄性Wistar大鼠通过饮用水接触镉（Cd，15ppm，持续8周）对肝脏脂质代谢的影响。与未接触金属（对照）的大鼠相比，血清甘油三酯、胆固醇以及低密度脂蛋白+极低密度脂蛋白胆固醇浓度升高。这与肝素化后血浆中脂蛋白脂肪酶活性降低有关。肝脏极低密度脂蛋白的分泌未发生改变。镉处理增加了肝脏中甘油三酯含量，降低了酯化胆固醇含量。高甘油三酯水平与3-磷酸甘油酰基转移酶mRNA表达增加有关。此外，通过脂肪酸合成酶和异柠檬酸脱氢酶活性的增加以及[（14）C]-乙酸掺入可皂化脂质部分测定，肝脏脂肪酸合成增加。与对照相比，棕榈酸（16:0）和总饱和脂肪酸的相对百分比增加。肝脏葡萄糖-6-磷酸脱氢酶、苹果酸脱氢酶和胆固醇酯水解酶活性未发生变化。在肝脏中，与对照相比，镉处理降低了线粒体中的甘油三酯和胆固醇含量，同时增加了细胞质中的甘油三酯含量以及细胞核中的胆固醇和磷脂含量。此外，观察到细胞核磷脂酰胆碱合成增加。镉暴露直接或间接改变血清脂质含量和肝脏脂质代谢。
