Pramod S N, Venkatesh Y P
Department of Biochemistry and Nutrition, Central Food Technological Research Institute, Mysore, India.
J Investig Allergol Clin Immunol. 2008;18(1):59-62.
We describe a case of allergy caused by ingestion of eggplant in an atopic subject. Symptoms included urticaria, itching of the throat, and hoarseness. Skin prick test (SPT) was positive with 4 varieties of eggplant; however, allergen-specific immunoglobulin E was not detected. SPT with fractions of green long eggplant extract obtained by dialysis and ultrafiltration suggested the allergen to be less than 10 kd. SPT following acetone precipitation of eggplant extract revealed that the allergen was present in the supernatant portion. Further analysis by size-exclusion chromatography of the 10 kd filtrate of eggplant extract on Sephadex G-25 followed by SPT of fractions revealed that the causative allergen was a low molecular weight nonprotein secondary metabolite of less than 1 kd. To our knowledge, this is the first report of allergy to the ingestion of eggplant in which a nonprotein secondary metabolite has been detected as an allergen.
我们描述了一例特应性个体因摄入茄子而引发过敏的病例。症状包括荨麻疹、喉咙瘙痒和声音嘶哑。对4种茄子进行皮肤点刺试验（SPT）呈阳性；然而，未检测到过敏原特异性免疫球蛋白E。对通过透析和超滤获得的绿长茄子提取物组分进行SPT，表明过敏原分子量小于10kd。对茄子提取物进行丙酮沉淀后进行SPT，结果显示过敏原存在于上清液部分。通过葡聚糖凝胶G - 25对茄子提取物的10kd滤液进行尺寸排阻色谱进一步分析，随后对各组分进行SPT，结果表明致病过敏原是一种分子量小于1kd的低分子量非蛋白质次生代谢产物。据我们所知，这是首次报告摄入茄子过敏且检测到非蛋白质次生代谢产物作为过敏原的病例。
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