Yamakuchi Munekazu, Ferlito Marcella, Morrell Craig N, Matsushita Kenji, Fletcher Craig A, Cao Wangsen, Lowenstein Charles J
Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods Mol Biol. 2008;440:203-15. doi: 10.1007/978-1-59745-178-9_15.
Endothelial exocytosis of granules is a rapid response to vascular injury. However, the molecular machinery that regulates exocytosis in endothelial cells is not well understood. Recently developed techniques have defined the endothelial proteins that control vesicle and granule trafficking in endothelial cells. These techniques have revealed that syntaxin 4, synaptobrevin 3, and N-ethylmaleimide-sensitive factor (NSF) play a critical role in endothelial granule exocytosis. Additional studies have shown that nitric oxide regulates exocytosis by chemically modifying NSF. Further characterization of the factors that regulate exocytosis will lead to novel treatments for vascular diseases such as myocardial infarction and stroke.
内皮细胞颗粒的胞吐作用是对血管损伤的快速反应。然而,调节内皮细胞胞吐作用的分子机制尚未完全明确。最近开发的技术已确定了在内皮细胞中控制囊泡和颗粒运输的内皮蛋白。这些技术表明, syntaxin 4、突触小泡蛋白3和N - 乙基马来酰亚胺敏感因子(NSF)在内皮颗粒胞吐作用中起关键作用。进一步的研究表明,一氧化氮通过化学修饰NSF来调节胞吐作用。对调节胞吐作用的因素进行进一步表征将为心肌梗死和中风等血管疾病带来新的治疗方法。