隐孢子虫脱囊过程中生命周期各阶段的观察

Observations on Cryptosporidium life cycle stages during excystation.

作者信息

Karanis Panagiotis, Kimura Akio, Nagasawa Hideyuki, Igarashi Ikuo, Suzuki Naoyoshi

机构信息

Medical and Molecular Parasitology Laboratory, Medical School, Center of Anatomy, Institute II, University of Cologne, 50937 Cologne, Germany.

出版信息

J Parasitol. 2008 Feb;94(1):298-300. doi: 10.1645/GE-1185.1.

DOI:10.1645/GE-1185.1

PMID:18372656

Abstract

Cryptosporidium parvum (HNJ-1 strain, genotype 2) merozoites were released from oocysts directly during an incubation and excystation procedure without bleach treatment. They were polymorphic, mostly spindle-shaped; others were bean shaped, actively motile, and underwent division. Merozoites survived for short time-period in an in vitro culture system, but could not be established in a subsequent cultivation effort in RPMI medium.

摘要

微小隐孢子虫（HNJ-1株，基因型2）裂殖子在无漂白剂处理的孵育和脱囊过程中直接从卵囊中释放出来。它们形态多样，大多呈纺锤形；其他的呈豆形，活动活跃，并进行分裂。裂殖子在体外培养系统中存活时间较短，但在随后用RPMI培养基进行的培养中未能成功培养。