基于铁氰化钾（III）氧化反应的固定化试剂化学发光流动传感器用于邻苯三酚的测定。

Chemiluminescence flow sensor with immobilized reagent for the determination of pyrogallol based on potassium hexacyanoferrate(III) oxidation.

作者信息

Chen Jianchun, Bai Jun

机构信息

Physical Education and Human Kinesiology Department, University of Science and Technology of Qingdao, Zhengzhou Road 53, Qingdao 266042, China.

出版信息

Spectrochim Acta A Mol Biomol Spectrosc. 2008 Dec 1;71(3):989-92. doi: 10.1016/j.saa.2008.02.022. Epub 2008 Feb 19.

DOI:10.1016/j.saa.2008.02.022

PMID:18373950

Abstract

A novel chemiluminescence (CL) flow-through sensor for the determination of pyrogallol has been developed. The method is based on the reaction between pyrogallol and potassium hexacyanoferrate(III) in sodium hydroxide solution. Potassium hexacyanoferrate(III) involved in the CL reaction was electrostatically immobilized on anion-exchange resin packed in a column. Pyrogallol was sensed by the CL reaction between pyrogallol and potassium hexacyanoferrate(III) which was eluted from the ion-exchange column through sodium phosphate injection. The CL emission allows quantitation of pyrogallol concentration in the range 0.01-3.8 microg/mL with a detection limit (3 sigma) of 0.003 microg/mL and a sample throughput of 118 h(-1). The relative standard deviation (n=7) was 2.2% for 0.2 microg/mL of pyrogallol. The influence of foreign compounds was tested.

摘要

一种用于测定连苯三酚的新型化学发光（CL）流通式传感器已被开发出来。该方法基于连苯三酚与铁氰化钾（III）在氢氧化钠溶液中的反应。参与CL反应的铁氰化钾（III）通过静电作用固定在填充于柱中的阴离子交换树脂上。通过磷酸钠进样从离子交换柱中洗脱出来的连苯三酚与铁氰化钾（III）之间的CL反应来检测连苯三酚。CL发射使得能够对浓度在0.01 - 3.8微克/毫升范围内的连苯三酚进行定量，检测限（3σ）为0.003微克/毫升，样品通量为118小时-1。对于0.2微克/毫升的连苯三酚，相对标准偏差（n = 7）为2.2%。测试了外来化合物的影响。