[人巨噬细胞金属弹性蛋白酶mRNA在胃癌细胞系及组织中的表达及其临床意义]

[The expression of human macrophage metalloelastase mRNA in gastric cancer cell lines and tissues and its clinical significance].

作者信息

Zhang Hong, Li You-Ming, Xu Guo-Qiang

机构信息

Department of Gastroenterology, First Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310003, China.

出版信息

Zhonghua Zhong Liu Za Zhi. 2007 Nov;29(11):830-2.

PMID:18396640

Abstract

OBJECTIVE

To make measurement of the expression of HME mRNA in both gastric cancer cell lines and tissues and evaluate its role in development of gastric cancer.

METHODS

The HME mRNA expression in 3 gastric cancer cell lines and tissues was detected by real-time fluorescent quantitative PCR.

RESULTS

All three gastric cancer cell lines expressed HME mRNA, and the HME mRNA expression level in gastric cancer tissues was higher than that in normal tissues (P <0.05). The rate of lymph node metastasis of HME mRNA positive cases was lower than that of HME mRNA negative ones (P < 0.05). Expression of drug resistance gene GST of HME mRNA positive cases was lower than that of HME mRNA negative ones (P <0.05). The two-year survival rate of HME mRNA positive cases was higher than that of HME mRNA negative ones (P < 0.05). There was no correlation between the expression of HME gene and the tumor location, size, depth of invasion, degree of malignancy, expression of drug resistance gene top II and PG.

CONCLUSION

The expression of HME gene in gastric cancers may be related with lower possibility of metastasis and predict a better prognosis.

摘要

目的

检测胃癌细胞系及组织中HME mRNA的表达水平，并评估其在胃癌发生发展中的作用。

方法

采用实时荧光定量PCR检测3种胃癌细胞系及组织中HME mRNA的表达。

结果

3种胃癌细胞系均表达HME mRNA，胃癌组织中HME mRNA表达水平高于正常组织（P<0.05）。HME mRNA阳性病例的淋巴结转移率低于HME mRNA阴性病例（P<0.05）。HME mRNA阳性病例的耐药基因GST表达低于HME mRNA阴性病例（P<0.05）。HME mRNA阳性病例的2年生存率高于HME mRNA阴性病例（P<0.05）。HME基因表达与肿瘤部位、大小、浸润深度、恶性程度、耐药基因top II及PG表达均无相关性。