嗜热栖热放线菌色氨酸合成酶 I 基因（trpE）的核苷酸序列。
Nucleotide sequence of trpE, anthranilate synthase I gene, of Bacillus caldotenax.
作者信息
Shiratsuchi A, Sato S
机构信息
Mitsubishi-Kasei Institute of Life Sciences, Tokyo, Japan.
出版信息
Biochim Biophys Acta. 1991 Nov 11;1090(3):348-50. doi: 10.1016/0167-4781(91)90201-v.
PMID:1840498
Abstract
trpE gene of Bacillus caldotenax was cloned by complementation of an Escherichia coli tryptophan auxotroph. The trpE encoding a 508 amino acid polypeptide is followed by trpD. S1-nuclease mapping of a trpE in vivo transcript suggested that a promoter preceding the trpE was utilized by E. coli. The deduced amino acid sequence of anthranilate synthase I (ASI) was compared with that of five other bacterials ASIs.
摘要
通过对大肠杆菌色氨酸营养缺陷型进行互补，克隆了嗜热栖热芽孢杆菌的trpE基因。编码508个氨基酸多肽的trpE后面是trpD。对trpE体内转录本进行S1核酸酶图谱分析表明，trpE之前的一个启动子被大肠杆菌所利用。将邻氨基苯甲酸合酶I（ASI）的推导氨基酸序列与其他五种细菌的ASI进行了比较。
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