土耳其2型糖尿病伴或不伴肾病患者血管紧张素原M235T和血管紧张素转换酶插入/缺失基因多态性的关联

Association of the angiotensinogen M235T and angiotensin-converting enzyme insertion/deletion gene polymorphisms in Turkish type 2 diabetic patients with and without nephropathy.

作者信息

Eroglu Zuhal, Cetinkalp S, Erdogan Mehmet, Kosova B, Karadeniz Muammer, Kutukculer A, Gunduz C, Tetik A, Topcuoglu N, Ozgen A G, Tuzun M

机构信息

Department of Medical Biology, Medical School, University Ege, Izmir, Turkey.

出版信息

J Diabetes Complications. 2008 May-Jun;22(3):186-90. doi: 10.1016/j.jdiacomp.2006.12.004. Epub 2008 Apr 16.

DOI:10.1016/j.jdiacomp.2006.12.004

PMID:18413162

Abstract

OBJECTIVE

Recent studies have suggested an association between a deletion variant of the angiotensin-converting enzyme (ACE) gene and diabetic nephropathy. However, this finding has not been confirmed by all investigators. Furthermore, an M235T variant of the angiotensinogen (AGT) gene has been associated with hypertension, an important risk factor for the development and progression of diabetic nephropathy.

RESEARCH DESIGN AND METHODS

We investigated the relationship of the ACE insertion/deletion (I/D) and AGT M235T gene polymorphisms in Turkish patients with type 2 diabetes mellitus (DM) with and without diabetic nephropathy. A total of 102 individuals were screened for the presence of the ACE I/D and AGT M235T polymorphism: 46 individuals who had type 2 DM with diabetic nephropathy and, as controls, 56 individuals who had type 2 DM without diabetic nephropathy. Gene polymorphisms were determined by the specific melting temperature (T(m)) values of the resulting amplicons after real-time online polymerase chain reaction and melting curve analysis.

RESULTS

The frequencies of the ACE DD, ID, and II genotypes were 34.8%, 37.0%, and 28.3%, respectively, among type 2 diabetic patients with nephropathy, and 33.9%, 42.9%, 23.2%, respectively (P=.788), in the control subjects without diabetic nephropathy. On the other hand, the frequencies of the AGT MM, MT, and TT genotypes among the same groups were 26.1%, 52.2%, 21.7% and 26.8%, 57.1%, 16.1%, respectively (P=.758).

CONCLUSIONS

There were no differences in the frequencies of the AGT M235T and ACE I/D genotypes between Turkish patients with type 2 DM with and without nephropathy.

摘要

目的

近期研究提示血管紧张素转换酶（ACE）基因的缺失变异与糖尿病肾病之间存在关联。然而，并非所有研究者均证实了这一发现。此外，血管紧张素原（AGT）基因的M235T变异与高血压相关，而高血压是糖尿病肾病发生和进展的重要危险因素。

研究设计与方法

我们调查了土耳其2型糖尿病（DM）患者中，伴或不伴糖尿病肾病的ACE插入/缺失（I/D）和AGT M235T基因多态性之间的关系。共对102例个体进行了ACE I/D和AGT M235T多态性筛查：46例患有2型糖尿病合并糖尿病肾病的个体，以及作为对照的56例患有2型糖尿病但无糖尿病肾病的个体。通过实时在线聚合酶链反应和熔解曲线分析后，根据所得扩增子的特异性熔解温度（T(m)）值确定基因多态性。

结果

在患有肾病的2型糖尿病患者中，ACE DD、ID和II基因型的频率分别为34.8%、37.0%和28.3%，而在无糖尿病肾病的对照受试者中分别为33.9%、42.9%、23.2%（P = 0.788）。另一方面，同一组中AGT MM、MT和TT基因型的频率分别为26.1%、52.2%、21.7%和26.8%、57.1%、16.1%（P = 0.758）。