Rosu Valentina, Ahmed Niyaz, Paccagnini Daniela, Pacifico Adolfo, Zanetti Stefania, Sechi Leonardo A
Dipartimento di Scienze Biomediche, Sezione di Microbiologia clinica e sperimentale, viale San Pietro 43 b 07100 Sassari, Italy.
Ann Clin Microbiol Antimicrob. 2008 Apr 22;7:9. doi: 10.1186/1476-0711-7-9.
The role of pathogenic mycobacteria in diabetes has been a focus of speculation since a decade without any meaningful insights into the mechanism of diabetes causation vis a vis mycobacterial factors. Two of our studies based on PCR identification of mycobacterial DNA and detection of antibodies specific to the recombinant antigens and whole cell lysates of the Mycobacterium avium subsp. paratuberculosis (MAP) shown a clear association of MAP with the presence of type 1 diabetes mellitus (T1DM).
In this study, we sought to investigate if or not type 2 diabetes (T2DM) patients harbour humoral responses to MAP. Using three different MAP antigen preparations, humoral antibody profiles were estimated for 57 T2DM patients and 57 healthy controls. Statistical analysis was performed with the Chi-square test with Yates' corrections.
We observed insignificant levels of humoral antibodies against recombinant heparin binding haemagglutinin (HbHA), glycosyl transferase (Gsd) and MAP whole cell lysate in the blood of subjects with T2DM as compared to healthy controls.
We found no obvious association of MAP with the incidence of T2DM in Sardinian patients.
自十年前以来，致病性分枝杆菌在糖尿病中的作用一直是推测的焦点，但对于糖尿病病因与分枝杆菌因素之间的机制尚无任何有意义的见解。我们基于聚合酶链反应（PCR）鉴定分枝杆菌DNA以及检测鸟分枝杆菌副结核亚种（MAP）重组抗原和全细胞裂解物特异性抗体的两项研究表明，MAP与1型糖尿病（T1DM）的存在存在明显关联。
在本研究中，我们试图调查2型糖尿病（T2DM）患者是否对MAP产生体液免疫反应。使用三种不同的MAP抗原制剂，对57例T2DM患者和57例健康对照者的体液抗体谱进行了评估。采用经Yates校正的卡方检验进行统计分析。
与健康对照相比，我们观察到T2DM患者血液中针对重组肝素结合血凝素（HbHA）、糖基转移酶（Gsd）和MAP全细胞裂解物的体液抗体水平无显著差异。
我们发现，在撒丁岛患者中，MAP与T2DM的发病率无明显关联。
