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火鸡感染传染性喉气管炎病毒的自然感染。

Natural infection of turkeys by infectious laryngotracheitis virus.

作者信息

Portz Cristiana, Beltrão Nilzane, Furian Thales Quedi, Júnior Alfredo Bianco, Macagnan Marisa, Griebeler Josiane, Lima Rosa Carlos André Veiga, Colodel Edson Moleta, Driemeier David, Back Alberto, Barth Schatzmayr Ortrud Monika, Canal Cláudio Wageck

机构信息

Laboratório de Virologia, Faculdade de Veterinária da UFRGS, Av. Bento Gonçalves, 9090 Bairro Agronomia, Porto Alegre, Brazil.

出版信息

Vet Microbiol. 2008 Sep 18;131(1-2):57-64. doi: 10.1016/j.vetmic.2008.02.029. Epub 2008 Mar 18.

DOI:10.1016/j.vetmic.2008.02.029
PMID:18436397
Abstract

The infectious laryngotracheitis virus (ILTV) is an important respiratory pathogen of chickens that also infects pheasants and peafowl. Epidemiologically non-related commercial turkey flocks with clinical signs such as tracheitis, swollen sinuses, conjunctivitis and expectoration of bloody mucus were examined for the presence of the virus. Laboratory ILTV detection was performed by virus isolation in embryonated eggs and cell cultures, PCR and sequencing of amplification products, histopathology, indirect immunofluorescence and electron microscopy. One ILTV turkey isolate was also experimentally inoculated into susceptible chickens and turkeys, reproducing a mild respiratory disease. This is the first description of natural infections with ILTV in turkeys.

摘要

传染性喉气管炎病毒(ILTV)是鸡的一种重要呼吸道病原体,也可感染雉鸡和孔雀。对出现气管炎症、鼻窦肿胀、结膜炎和咳出带血黏液等临床症状但在流行病学上无关联的商品火鸡群进行了病毒检测。通过在鸡胚和细胞培养物中进行病毒分离、对扩增产物进行聚合酶链反应(PCR)和测序、组织病理学、间接免疫荧光和电子显微镜检查等方法进行实验室ILTV检测。还将一株ILTV火鸡分离株经实验接种到易感鸡和火鸡中,引发了轻度呼吸道疾病。这是首次对火鸡自然感染ILTV的描述。

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