[胶体金标记免疫层析试纸条快速诊断疟疾的建立与评价]

[Establishment and evaluation of colloid gold labeled immunochromatographic strip test for rapid diagnosis of malaria].

作者信息

Wang Jun-yun, Shi Feng, Yang Yue-tao, Gao Chun-hua, Bao Yi-fang, Tang Lin-hua

机构信息

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2007 Oct;25(5):415-8.

PMID:18441999

Abstract

OBJECTIVE

To establish and evaluate a gold immunochromatographic strip test for detection and differentiation of Plasmodium vivax and P. falciparum.

METHODS

The monoclonal antibodies, F4H12, G4C9 and D8F7, were conjugated with colloid gold as detecting reagent; monoclonal antibody B2G10 (against P. vivax/ P. falciparum) and D6A7 (only against P. falciparum) were immobilized on nitrocellulose in proper position. Blood samples from 107 febrile patients from endemic area of malaria and 17 patients with visceral leishmaniasis were used for evaluating the specificity. Blood samples of malaria patients (110 with P. vivax and 54 with P. falciparum) were used for evaluating the sensitivity.

RESULTS

5 samples out of 107 febrile patients and 17 patients with visceral leishmaniasis showed false positive reaction with a specificity of 96.0% (119/124), all the 17 samples from patients with visceral leishmaniasis were negative. 164 blood samples of malaria patients showed a sensitivity of 92.3% (153/164), 92.7% (102/110)and 94.4% (51/ 54) for patients infected with P. vivax or P. falciparum, respectively.

CONCLUSION

The immunochromatographic strip test based on antigen-capturing is a sensitive, specific, simple and rapid assay for malaria diagnosis.

摘要

目的

建立并评估一种用于检测和鉴别间日疟原虫与恶性疟原虫的金免疫层析试纸条检测法。

方法

将单克隆抗体F4H12、G4C9和D8F7与胶体金偶联作为检测试剂；将单克隆抗体B2G10（抗间日疟原虫/恶性疟原虫）和D6A7（仅抗恶性疟原虫）固定于硝酸纤维素膜的适当位置。采用来自疟疾流行区的107例发热患者和17例内脏利什曼病患者的血样评估特异性。采用疟疾患者的血样（110例间日疟患者和54例恶性疟患者）评估敏感性。