采用响应面法优化脂肪酶催化合成丙烯酸山梨醇酯的工艺

Optimization of lipase-catalyzed synthesis of sorbitan acrylate using response surface methodology.

作者信息

Jeong Gwi-Taek, Park Don-Hee

机构信息

School of Biological Sciences and Technology, Chonnam National University, 500-757, Gwangju, Korea.

出版信息

Appl Biochem Biotechnol. 2007 Apr;137-140(1-12):595-609. doi: 10.1007/s12010-007-9082-y.

DOI:10.1007/s12010-007-9082-y

PMID:18478419

Abstract

In this study, we have synthesized sorbitan acrylate through response surface methodology, using sorbitan and vinyl acrylate that catalyze immobilized lipase. In order to optimize the enzymatic synthesis of the sorbitan acrylate, we applied response surface techniques to determine the effects of five-level-four-factors and their reciprocal interactions with the biosynthesis of sorbitan acrylate. Our statistical model predicted that the highest conversion yield of sorbitan acrylate would be approx 100%, under the following optimized reaction conditions: a reaction temperature of 40.1 degrees C, a reaction time of 237.4 min, an enzyme concentration of 8%, and a 4.49:1 acyl donor/acceptor molar ratio. Using these optimal conditions in three independent replicates, the conversion yield reached 97.6+/-1.3%.

摘要

在本研究中，我们采用响应面法，以脱水山梨醇和丙烯酸乙烯酯为原料，利用固定化脂肪酶作为催化剂，合成了丙烯酸山梨醇酯。为了优化丙烯酸山梨醇酯的酶促合成，我们应用响应面技术来确定五水平四因素及其相互作用对丙烯酸山梨醇酯生物合成的影响。我们的统计模型预测，在以下优化反应条件下，丙烯酸山梨醇酯的最高转化率约为100%：反应温度40.1℃，反应时间237.4分钟，酶浓度8%，酰基供体/受体摩尔比4.49:1。在三个独立重复实验中使用这些最佳条件，转化率达到97.6±1.3%。