过氧亚硝酸根介导硫酸双肼屈嗪二氧化碳增强氧化反应的化学发光研究及其在药物分析中的应用

Chemiluminescence investigation of carbon dioxide-enhanced oxidation of dihydralazine sulfate by peroxynitrite and its application to pharmaceutical analysis.

作者信息

Yang Xiao-Feng

机构信息

Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, Institute of Analytical Science, Department of Chemistry, Northwest University, Taibai Road #229, Xi'an 710069, China.

出版信息

Anal Chim Acta. 2008 Jun 2;616(2):190-5. doi: 10.1016/j.aca.2008.04.032. Epub 2008 Apr 20.

DOI:10.1016/j.aca.2008.04.032

PMID:18482603

Abstract

A weak chemiluminescence (CL) emission was observed upon mixing peroxynitrite (ONOO(-)) with dihydralazine sulfate (DHZS). Further experiments showed that carbonate media could enhance the CL emission significantly. Based on these observations, a novel flow injection CL method for the determination of DHZS is developed. The CL signal is linearly with DHZS concentration in the range of 0.01-3.0 microg mL(-1) with a detection limit of 3.6 ng mL(-1). The method was applied to the analysis of DHZS in pharmaceutical preparations and compared well with the high-performance liquid chromatography (HPLC) method. The CL mechanism is discussed and it is postulated that it involves nitrosoperoxocarboxylate (ONOOCO(2)(-)), which is an unstable adduct and can rapidly decompose into *NO(2) and *CO(3)(-) radical. The latter can then oxidize DHZS to give out strong CL emission.

摘要

将过氧亚硝酸盐（ONOO(-)）与硫酸双肼屈嗪（DHZS）混合时观察到微弱的化学发光（CL）发射。进一步的实验表明，碳酸盐介质可显著增强CL发射。基于这些观察结果，开发了一种用于测定DHZS的新型流动注射CL方法。CL信号与DHZS浓度在0.01 - 3.0 μg mL(-1)范围内呈线性关系，检测限为3.6 ng mL(-1)。该方法应用于药物制剂中DHZS的分析，与高效液相色谱（HPLC）法相比效果良好。讨论了CL机制，并推测其涉及亚硝基过氧羧酸盐（ONOOCO(2)(-)），它是一种不稳定的加合物，可迅速分解为NO(2)和CO(3)(-)自由基。后者随后可氧化DHZS以发出强烈的CL发射。