College of Animal science, South China Agricultural University, Guangzhou, China.
J Anim Physiol Anim Nutr (Berl). 2009 Aug;93(4):439-46. doi: 10.1111/j.1439-0396.2008.00825.x.
Fatty acid transport protein 1 (FATP-1) is a membrane associated protein, which facilitates the long chain fatty acids (LCFA) transport across the plasma membrane for the LCFA utilization and storage. In this study, the cDNA structure of porcine FATP-1 was investigated and the gene expression patterns of porcine FATP-1 in different tissues were tested by RT-PCR and Southern blot analysis. The results showed that there were five pFATP-1 mRNA species, namely, FATP-1a, FATP-1aV, FATP-1b, FATP-1c and FATP-1cV and are generated by alternative splicing of primary transcript. Deduced pFATP-1a protein showed 91.6% and 87.5% identities with those of human and rat. RT-PCR and Southern blot analysis demonstrated widespread tissue distribution of each pFATP-1 isoform mRNA, most abundantly in the brain, heart, lung, jejunum, testis, pancreas and trapezius muscle. Real-time quantitative RT-PCR revealed that pFATP-1 mRNA expressions in masseter and trapezius muscles were much higher than those in longissimus, gluteus medius and adipose tissues. These results suggested a crucial physiological role of pFATP-1 in fatty acid utilization in muscles, especially red muscles tissues, rather than fat storage in adipose tissues.
脂肪酸转运蛋白 1（FATP-1）是一种膜相关蛋白，它促进长链脂肪酸（LCFA）穿过质膜，以便 LCFA 的利用和储存。在这项研究中，我们研究了猪 FATP-1 的 cDNA 结构，并通过 RT-PCR 和 Southern blot 分析检测了猪 FATP-1 在不同组织中的基因表达模式。结果表明，存在 5 种 pFATP-1 mRNA 物种，即 FATP-1a、FATP-1aV、FATP-1b、FATP-1c 和 FATP-1cV，它们是通过初级转录物的选择性剪接产生的。推导的 pFATP-1a 蛋白与人源和鼠源的蛋白具有 91.6%和 87.5%的同一性。RT-PCR 和 Southern blot 分析表明，每种 pFATP-1 同工型 mRNA 在广泛的组织中分布，在脑、心、肺、空肠、睾丸、胰腺和斜方肌中最为丰富。实时定量 RT-PCR 显示，在咀嚼肌和斜方肌中的 pFATP-1 mRNA 表达水平明显高于在长肌、臀中肌和脂肪组织中的表达水平。这些结果表明，pFATP-1 在肌肉（尤其是红肌组织）中脂肪酸的利用中具有重要的生理作用，而不是在脂肪组织中储存脂肪。
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