对一种未分类、无包涵体的DNA病毒（致家蝇唾液腺肥大病毒，MdSGHV）进行序列分析。
Sequence analysis of a non-classified, non-occluded DNA virus that causes salivary gland hypertrophy of Musca domestica, MdSGHV.
作者信息
Garcia-Maruniak Alejandra, Maruniak James E, Farmerie William, Boucias Drion G
机构信息
Department of Entomology and Nematology, PO Box 110620, University of Florida, Gainesville, Florida 32611-0620, USA.
出版信息
Virology. 2008 Jul 20;377(1):184-96. doi: 10.1016/j.virol.2008.04.010. Epub 2008 May 21.
The genome of the virus that causes salivary gland hypertrophy in Musca domestica (MdSGHV) was sequenced. This non-classified, enveloped, double stranded, circular DNA virus had a 124,279bp genome. The G + C content was 43.5% with 108 putative methionine-initiated open reading frames (ORFs). Thirty ORFs had homology to database proteins: eleven to proteins coded by both baculoviruses and nudiviruses (p74, pif-1, pif-2, pif-3, odv-e66, rr1, rr2, iap, dUTPase, MMP, and Ac81-like), seven to nudiviruses (mcp, dhfr, ts, tk and three unknown proteins), one to baculovirus (Ac150-like), one to herpesvirus (dna pol), and ten to cellular proteins. Mass spectrum analysis of the viral particles' protein components identified 29 structural ORFs, with only p74 and odv-e66 previously characterized as baculovirus structural proteins. Although most of the homology observed was to nudiviruses, phylogenetic analysis showed that MdSGHV was not closely related to them or to the baculoviruses.
对导致家蝇唾液腺肥大的病毒（MdSGHV）的基因组进行了测序。这种未分类的、有包膜的双链环状DNA病毒基因组大小为124,279bp。G + C含量为43.5%，有108个推定的甲硫氨酸起始开放阅读框（ORF）。30个ORF与数据库蛋白具有同源性：11个与杆状病毒和浓核病毒编码的蛋白同源（p74、pif-1、pif-2、pif-3、odv-e66、rr1、rr2、iap、dUTPase、MMP和Ac81样蛋白），7个与浓核病毒同源（mcp、dhfr、ts、tk和3个未知蛋白），1个与杆状病毒同源（Ac150样蛋白），1个与疱疹病毒同源（dna pol），10个与细胞蛋白同源。对病毒颗粒蛋白成分的质谱分析确定了29个结构ORF，其中只有p74和odv-e66以前被鉴定为杆状病毒结构蛋白。尽管观察到的大多数同源性是与浓核病毒，但系统发育分析表明MdSGHV与它们或杆状病毒没有密切关系。
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