Xue Yan, Tong Xiang-Hui, Wang Feng, Zhao Wei-Guo
Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Yao Xue Xue Bao. 2008 Feb;43(2):221-3.
To develop an UPLC-UV method for the determination of aristolochic acid A in the aerial and underground parts of Asarum, an AcquityUPLC HSST3 column (50 mm x 2.1 mm ID, 1.8 microm) was used with the mobile phase of acetonitrile-0.1% FA and eluted in gradient mode. The flow rate was 0.5 mL min(-1), the detection wavelength was 251 nm, and the column temperature was 40 degrees C. The calibration curve was linear in the range of 18 - 1450 ng of aristolochic acid A with correlation coefficient of 0. 9999 and the minimum detective concentration was 18.25 ng mL (-1). The method developed is accurate and reproducible, and can be applied in the determination of aristolochic acid A in Asarum.
为建立一种用于测定细辛地上和地下部分中马兜铃酸A的超高效液相色谱-紫外检测方法，采用AcquityUPLC HSST3色谱柱（50 mm×2.1 mm内径，1.8微米），以乙腈-0.1%甲酸为流动相，梯度洗脱。流速为0.5 mL min(-1)，检测波长为251 nm，柱温为40℃。马兜铃酸A在18 - 1450 ng范围内校准曲线呈线性，相关系数为0.9999，最低检测浓度为18.25 ng mL(-1)。所建立的方法准确、可重复，可用于细辛中马兜铃酸A的测定。
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