临床局限性前列腺癌的基因谱鉴定

Genetic profile identification in clinically localized prostate carcinoma.

作者信息

Gallucci Michele, Merola Roberta, Leonardo Costantino, De Carli Piero, Farsetti Antonella, Sentinelli Steno, Sperduti Isabella, Mottolese Marcella, Carlini Paolo, Vico Erika, Simone Giuseppe, Cianciulli Annamaria

机构信息

Department of Urology, Regina Elena Cancer Institute, Rome, Italy.

出版信息

Urol Oncol. 2009 Sep-Oct;27(5):502-8. doi: 10.1016/j.urolonc.2008.04.008. Epub 2008 Jun 4.

DOI:10.1016/j.urolonc.2008.04.008

PMID:18534873

Abstract

PURPOSE

To confirm our previously obtained results, we genetically characterized prostate cancer from patients undergo radical prostatectomy in a retrospective study.

MATERIALS AND METHODS

Histological sections were evaluated for 106 patients treated with surgery from 1991 to 2004. With fluorescence in situ hybridization (FISH) method, the status of LPL (8p22), c-MYC (8q24) genes and 7, 8, X chromosomes was evaluated.

RESULTS

Chromosomes 7, 8, X aneusomy was demonstrated in 91.5%, 78.3%, and 51.9% of the samples, respectively, whereas LPL deletion and MYC amplification were found in 76.0% and 1.6%. A genetic profile was considered as unfavorable when at least two aneusomic chromosomes and one altered gene were present. Tumors with an adverse genetic profile were more frequently present in patients with higher stages (P = 0.02), biochemical/clinical progression (P = 0.03), and Gleason grade 4 + 3 (P = 0.02). Multiple correspondence analysis identified one tumor group characterized by chromosome 8 aneusomy, X polysomy, LPL gene deletion, Gleason > 7 and 4 + 3 associated with progression.

CONCLUSIONS

In this study, we recognized the predictive power of previously identified cytogenetic profiles. Assessment of genetic set may characterize each patient and have influence on postoperative therapeutic strategies.

摘要

目的

为了证实我们之前获得的结果，我们在一项回顾性研究中对接受根治性前列腺切除术患者的前列腺癌进行了基因特征分析。

材料与方法

对1991年至2004年接受手术治疗的106例患者的组织学切片进行评估。采用荧光原位杂交（FISH）方法，评估脂蛋白脂肪酶（LPL，8p22）、原癌基因c-MYC（8q24）基因以及7号、8号和X染色体的状态。

结果

样本中7号、8号和X染色体三体性分别在91.5%、78.3%和51.9%的样本中得到证实，而LPL缺失和MYC扩增分别在76.0%和1.6%的样本中被发现。当存在至少两条三体性染色体和一个改变的基因时，基因图谱被认为是不利的。具有不良基因图谱的肿瘤在更高分期（P = 0.02）、生化/临床进展（P = 0.03）和 Gleason 分级4 + 3（P = 0.02）的患者中更常见。多重对应分析确定了一个肿瘤组，其特征为8号染色体三体性、X染色体多体性、LPL基因缺失、Gleason评分>7以及与进展相关的4 + 3。