利用5S rRNA基因间隔区鉴别川西獐牙菜及其混淆品獐牙菜属植物
Identification of Swertia mussotii and its adulterant Swertia species by 5S rRNA gene spacer.
作者信息
Yu Man-Tang, Wong Ka-Lok, Zong Yu-Ying, Shaw Pang-Chui, Che Chun-Tao
机构信息
Qinghai High Altitude Academy of Medicine and Science, Xining 810012, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Mar;33(5):502-4.
OBJECTIVE
This research focused on analyzing the differences of 5S rRNA gene spacer sequences on Swertia mussotii and its commonly used adulterants, including S. franchetiana, S. wolfangiana and S. chirayita.
METHOD
DNA was extracted from the collected Swertia samples. 5S rRNA intergenic spacers were amplified by PCR, sequenced and analyzed.
RESULT
5S rRNA gene spacer sequences were different between S. mussotii and its other three adulterants. Sequence divergence among species ranged from 30.6% to 65.0%.
CONCLUSION
5S rRNA spacers may be used as molecular authentication markers to differentiate S. mussotii and other commonly used Swertia adulterants. This result provides reliable and simple reference for the authentication of Swertia genus species.
目的
本研究着重分析川西獐牙菜及其常用混伪品（包括川西獐牙菜、紫红獐牙菜和印度獐牙菜）5S rRNA基因间隔序列的差异。
方法
从采集的獐牙菜样本中提取DNA。通过PCR扩增5S rRNA基因间隔区，进行测序和分析。
结果
川西獐牙菜与其其他三种混伪品之间的5S rRNA基因间隔序列不同。物种间的序列差异范围为30.6%至65.0%。
结论
5S rRNA间隔区可作为分子鉴定标记来区分川西獐牙菜和其他常用的獐牙菜混伪品。该结果为獐牙菜属物种的鉴定提供了可靠且简便的参考。
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